Altered amyloid precursor protein processing regulates glucose uptake and oxidation in cultured rodent myotubes.

ABSTRACT:

Aims/hypothesis

Impaired glucose uptake in skeletal muscle is an important contributor to glucose intolerance in type 2 diabetes. The aspartate protease, beta-site APP-cleaving enzyme 1 (BACE1), a critical regulator of amyloid precursor protein (APP) processing, modulates in vivo glucose disposal and insulin sensitivity in mice. Insulin-independent pathways to stimulate glucose uptake and GLUT4 translocation may offer alternative therapeutic avenues for the treatment of diabetes. We therefore addressed whether BACE1 activity, via APP processing, in skeletal muscle modifies glucose uptake and oxidation independently of insulin.

Methods

Skeletal muscle cell lines were used to investigate the effects of BACE1 and α-secretase inhibition and BACE1 and APP overexpression on glucos

SUBMITTER: Hamilton DL 

PROVIDER: S-EPMC4079947 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature