Genome-wide analysis of alternative splicing of pre-mRNA under salt stress in Arabidopsis.

ABSTRACT:

Background

Alternative splicing (AS) of precursor mRNA (pre-mRNA) is an important gene regulation process that potentially regulates many physiological processes in plants, including the response to abiotic stresses such as salt stress.

Results

To analyze global changes in AS under salt stress, we obtained high-coverage (~200 times) RNA sequencing data from Arabidopsis thaliana seedlings that were treated with different concentrations of NaCl. We detected that ~49% of all intron-containing genes were alternatively spliced under salt stress, 10% of which experienced significant differential alternative splicing (DAS). Furthermore, AS increased significantly under salt stress compared with under unstressed conditions. We demonstrated that most DAS genes were not differentially

SUBMITTER: Ding F 

PROVIDER: S-EPMC4079960 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature