Plasma microRNA expression and micronuclei frequency in workers exposed to polycyclic aromatic hydrocarbons.

ABSTRACT:

Background

Ubiquitous polycyclic aromatic hydrocarbons (PAHs) have been shown to alter gene expression patterns and elevate micronuclei (MN) frequency, but the underlying mechanisms are largely unknown. MicroRNAs (miRNAs) are key gene regulators that may be influenced by PAH exposures and mediate their effects on MN frequency.

Objectives

We sought to identify PAH-associated miRNAs and evaluate their associations with MN frequency.

Methods

We performed a two-stage study in healthy male coke oven workers to identify miRNAs associated with PAH exposures quantified using urinary monohydroxy-PAHs and plasma benzo[a]pyrene-r-7,t-8,c-10-tetrahydrotetrol-albumin (BPDE-Alb) adducts. In the discovery stage, we used Solexa sequencing to test differences in miRNA expression profi

SUBMITTER: Deng Q 

PROVIDER: S-EPMC4080537 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature