Discovery of novel Schistosoma japonicum antigens using a targeted protein microarray approach.

ABSTRACT:

Background

Novel vaccine candidates against Schistosoma japonicum are required, and antigens present in the vulnerable larval developmental stage are attractive targets. Post-genomic technologies are now available which can contribute to such antigen discovery.

Methods

A schistosome-specific protein microarray was probed using the local antibody response against migrating larvae. Antigens were assessed for their novelty and predicted larval expression and host-exposed features. One antigen was further characterised and its sequence and structure were analysed in silico. Real-time polymerase chain reaction was used to analyse transcript expression throughout development, and immunoblotting and enzyme-linked immunosorbent assays employed to determine antigen recognition by ant

SUBMITTER: McWilliam HE 

PROVIDER: S-EPMC4080988 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature