Regulation of the nucleosome repeat length in vivo by the DNA sequence, protein concentrations and long-range interactions.

ABSTRACT: The nucleosome repeat length (NRL) is an integral chromatin property important for its biological functions. Recent experiments revealed several conflicting trends of the NRL dependence on the concentrations of histones and other architectural chromatin proteins, both in vitro and in vivo, but a systematic theoretical description of NRL as a function of DNA sequence and epigenetic determinants is currently lacking. To address this problem, we have performed an integrative biophysical and bioinformatics analysis in species ranging from yeast to frog to mouse where NRL was studied as a function of various parameters. We show that in simple eukaryotes such as yeast, a lower limit for the NRL value exists, determined by internucleosome interactions and remodeler action. For higher eukaryotes,

SUBMITTER: Beshnova DA 

PROVIDER: S-EPMC4081033 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature