A DNA-binding-site landscape and regulatory network analysis for NAC transcription factors in Arabidopsis thaliana.

ABSTRACT: Target gene identification for transcription factors is a prerequisite for the systems wide understanding of organismal behaviour. NAM-ATAF1/2-CUC2 (NAC) transcription factors are amongst the largest transcription factor families in plants, yet limited data exist from unbiased approaches to resolve the DNA-binding preferences of individual members. Here, we present a TF-target gene identification workflow based on the integration of novel protein binding microarray data with gene expression and multi-species promoter sequence conservation to identify the DNA-binding specificities and the gene regulatory networks of 12 NAC transcription factors. Our data offer specific single-base resolution fingerprints for most TFs studied and indicate that NAC DNA-binding specificities might be predicted

SUBMITTER: Lindemose S 

PROVIDER: S-EPMC4081100 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature