Transactivation of proto-oncogene c-Myc by hepatitis B virus transactivator MHBs^t167.

ABSTRACT: C-terminally truncated hepatitis B virus (HBV) middle size surface proteins (MHBs^t) has been shown to be a transcriptional activator and may be relevant to hepatocarcinogenesis by transactivating gene expression. In the present study, a pcDNA3.1(-)-MHBs^t167 vector coding for MHBs^t truncated at amino acid 167 (MHBs^t167) was constructed and transfected into the HepG2 hepatoma cell line. mRNA and protein expression of MHBs^t167 in the cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis. A cDNA library of genes transactivated by the truncated protein in HepG2 cells was made in pGEM-T Easy using suppression subtractive hybridization. The cDNAs were sequenced and analyzed with BLAST searchi

SUBMITTER: Lun YZ 

PROVIDER: S-EPMC4081436 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature