Versican regulates metastasis of epithelial ovarian carcinoma cells and spheroids.

ABSTRACT:

Background

Epithelial ovarian carcinoma is a deadly disease characterized by overt peritoneal metastasis. Individual cells and multicellular aggregates, or spheroids, seed these metastases, both commonly found in ascites. Mechanisms that foster spheroid attachment to the peritoneal tissues preceding formation of secondary lesions are largely unknown.

Methods

Cell culture models of SKOV-3, OVCAR3, OVCAR4, Caov-3, IGROV-1, and A2780 were used. In this report the role of versican was examined in adhesion of EOC spheroids and cells to peritoneal mesothelial cell monolayers in vitro as well as in formation of peritoneal tumors using an in vivo xenograft mouse model.

Results

The data demonstrate that versican is instrumental in facilitating cell and spheroid adhesion to the

SUBMITTER: Desjardins M 

PROVIDER: S-EPMC4081460 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature