Dual modifications strategy to quantify neutral and sialylated N-glycans simultaneously by MALDI-MS.

ABSTRACT: Differences in ionization efficiency among neutral and sialylated glycans prevent direct quantitative comparison by their respective mass spectrometric signals. To overcome this challenge, we developed an integrated chemical strategy, Dual Reactions for Analytical Glycomics (DRAG), to quantitatively compare neutral and sialylated glycans simultaneously by MALDI-MS. Initially, two glycan samples to be compared undergo reductive amination with 2-aminobenzoic acid and 2-(13)[C6]-aminobenzoic acid, respectively. The different isotope-incorporated glycans are then combined and subjected to the methylamidation of the sialic acid residues in one mixture, homogenizing the ionization responses for all neutral and sialylated glycans. By this approach, the expression change of relevant glycans betwee

SUBMITTER: Zhou H 

PROVIDER: S-EPMC4082391 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature