The 2HA line of Medicago truncatula has characteristics of an epigenetic mutant that is weakly ethylene insensitive.

ABSTRACT:

Background

The Medicago truncatula 2HA seed line is highly embryogenic while the parental line Jemalong rarely produces embryos. The 2HA line was developed from one of the rare Jemalong regenerates and this method for obtaining a highly regenerable genotype in M. truncatula is readily reproducible suggesting an epigenetic mechanism. Microarray transcriptomic analysis showed down regulation of an ETHYLENE INSENSITIVE 3-like gene in 2HA callus which provided an approach to investigating epigenetic regulation of genes related to ethylene signalling and the 2HA phenotype. Ethylene is involved in many developmental processes including somatic embryogenesis (SE) and is associated with stress responses.

Results

Microarray transcriptomic analysis showed a significant number of up-re

SUBMITTER: Kurdyukov S 

PROVIDER: S-EPMC4082419 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature