Differential contribution of monocytes to heart macrophages in steady-state and after myocardial infarction.

ABSTRACT:

Rationale

Macrophages populate the steady-state myocardium. Previously, all macrophages were thought to arise from monocytes; however, it emerged that, in several organs, tissue-resident macrophages may self-maintain through local proliferation.

Objective

Our aim was to study the contribution of monocytes to cardiac-resident macrophages in steady state, after macrophage depletion in CD11b(DTR/+) mice and in myocardial infarction.

Methods and results

Using in vivo fate mapping and flow cytometry, we estimated that during steady state the heart macrophage population turns over in ≈1 month. To explore the source of cardiac-resident macrophages, we joined the circulation of mice using parabiosis. After 6 weeks, we observed blood monocyte chimerism of 35.3±3.4%, whereas he

SUBMITTER: Heidt T 

PROVIDER: S-EPMC4082439 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature