Genome-wide identification of pathogenicity factors of the free-living amoeba Naegleria fowleri.

ABSTRACT:

Background

The free-living amoeba Naegleria fowleri is the causative agent of the rapidly progressing and typically fatal primary amoebic meningoencephalitis (PAM) in humans. Despite the devastating nature of this disease, which results in > 97% mortality, knowledge of the pathogenic mechanisms of the amoeba is incomplete. This work presents a comparative proteomic approach based on an experimental model in which the pathogenic potential of N. fowleri trophozoites is influenced by the compositions of different media.

Results

As a scaffold for proteomic analysis, we sequenced the genome and transcriptome of N. fowleri. Since the sequence similarity of the recently published genome of Naegleria gruberi was far lower than the close taxonomic relationship of these species would

SUBMITTER: Zysset-Burri DC 

PROVIDER: S-EPMC4082629 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature