Microfluidics embedded within extracellular matrix to define vascular architectures and pattern diffusive gradients.

ABSTRACT: Gradients of diffusive molecules within 3D extracellular matrix (ECM) are essential in guiding many processes such as development, angiogenesis, and cancer. The spatial distribution of factors that guide these processes is complex, dictated by the distribution and architecture of vasculature and presence of surrounding cells, which can serve as sources or sinks of factors. To generate temporally and spatially defined soluble gradients within a 3D cell culture environment, we developed an approach to patterning microfluidically ported microchannels that pass through a 3D ECM. Micromolded networks of sacrificial conduits ensconced within an ECM gel precursor solution are dissolved following ECM gelation to yield functional microfluidic channels. The dimensions and spatial layout of channels

SUBMITTER: Baker BM 

PROVIDER: S-EPMC4082768 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature