Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis.

ABSTRACT: Sulphur is an essential element for life and is ubiquitous in living systems. Yet how the sulphur atom is incorporated into many sulphur-containing secondary metabolites is poorly understood. For bond formation between carbon and sulphur in primary metabolites, the major ionic sulphur sources are the persulphide and thiocarboxylate groups on sulphur-carrier (donor) proteins. Each group is post-translationally generated through the action of a specific activating enzyme. In all reported bacterial cases, the gene encoding the enzyme that catalyses the carbon-sulphur bond formation reaction and that encoding the cognate sulphur-carrier protein exist in the same gene cluster. To study the production of the 2-thiosugar moiety in BE-7585A, an antibiotic from Amycolatopsis orientalis, we identifi

SUBMITTER: Sasaki E 

PROVIDER: S-EPMC4082789 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature