Direct control of type IIA topoisomerase activity by a chromosomally encoded regulatory protein.

ABSTRACT: Precise control of supercoiling homeostasis is critical to DNA-dependent processes such as gene expression, replication, and damage response. Topoisomerases are central regulators of DNA supercoiling commonly thought to act independently in the recognition and modulation of chromosome superstructure; however, recent evidence has indicated that cells tightly regulate topoisomerase activity to support chromosome dynamics, transcriptional response, and replicative events. How topoisomerase control is executed and linked to the internal status of a cell is poorly understood. To investigate these connections, we determined the structure of Escherichia coli gyrase, a type IIA topoisomerase bound to YacG, a recently identified chromosomally encoded inhibitor protein. Phylogenetic analyses indicat

SUBMITTER: Vos SM 

PROVIDER: S-EPMC4083091 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature