Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system.

ABSTRACT: Post-translational modification by SUMO is a highly conserved pathway in eukaryotes that plays very important regulatory roles in many cellular processes. Deregulation of the SUMO pathway contributes to the development and progression of many diseases including cancer. Therefore, identifying additional SUMO substrates and studying how their cellular and biological functions are regulated by sumoylation should provide new insights. Our studies showed that sumoylation activity was significant in Xenopus egg extracts, and that a high level of sumoylation was associated with sperm chromatin when SUMO was incubated with Xenopus egg extracts. By isolating SUMO-conjugated substrates using His-tagged SUMO1 or SUMO2 proteins under denaturing conditions, we identified 346 proteins by mass spectromet

SUBMITTER: Ma L 

PROVIDER: S-EPMC4083107 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature