Proteomic analysis of altered extracellular matrix turnover in bleomycin-induced pulmonary fibrosis.

ABSTRACT: Fibrotic disease is characterized by the pathological accumulation of extracellular matrix (ECM) proteins. Surprisingly, very little is known about the synthesis and degradation rates of the many proteins and proteoglycans that constitute healthy or pathological extracellular matrix. A comprehensive understanding of altered ECM protein synthesis and degradation during the onset and progression of fibrotic disease would be immensely valuable. We have developed a dynamic proteomics platform that quantifies the fractional synthesis rates of large numbers of proteins via stable isotope labeling and LC/MS-based mass isotopomer analysis. Here, we present the first broad analysis of ECM protein kinetics during the onset of experimental pulmonary fibrosis. Mice were labeled with heavy water for up

SUBMITTER: Decaris ML 

PROVIDER: S-EPMC4083112 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature