Efficient identification of mutated cancer antigens recognized by T cells associated with durable tumor regressions.

ABSTRACT:

Purpose

Cancer immunotherapy with adoptive transfer of tumor-infiltrating lymphocytes (TIL) represents an effective treatment for patients with metastatic melanoma, with the objective regressions in up to 72% of patients in three clinical trials. However, the antigen targets recognized by these effective TILs remain largely unclear.

Experimental design

Melanoma patients 2359 and 2591 both experienced durable complete regressions of metastases ongoing beyond five years following adoptive TIL transfer. Two conventional screening approaches were carried out to identify the antigens recognized by these clinically effective TILs. In addition, a novel approach was developed in this study to identify mutated T-cell antigens by screening a tandem minigene library, which comprised no

SUBMITTER: Lu YC 

PROVIDER: S-EPMC4083471 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature