Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cells.

ABSTRACT:

Background

The Src tyrosine kinase substrate and adaptor protein Tks5 had previously been implicated in the invasive phenotype of normal and transformed cell types via regulation of cytoskeletal structures called podosomes/invadopodia. The role of Src-Tks5 signaling in invasive prostate cancer, however, had not been previously evaluated.

Methods

We measured the relative expression of Tks5 in normal (n = 20) and cancerous (n = 184, from 92 patients) prostate tissue specimens by immunohistochemistry using a commercially available tumor microarray. We also manipulated the expression and activity of wild-type and mutant Src and Tks5 constructs in the LNCaP and PC-3 prostate cancer cell lines in order to ascertain the role of Src-Tks5 signaling in invadopodia development, matrix-

SUBMITTER: Burger KL 

PROVIDER: S-EPMC4083496 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature