Vasculogenesis in experimental stroke after human cerebral endothelial cell transplantation.

ABSTRACT:

Background and purpose

Despite the reported functional recovery in transplanted stroke models and patients, the mechanism of action underlying stem cell therapy remains not well understood. Here, we examined the role of stem cell-mediated vascular repair in stroke.

Methods

Adult rats were exposed to transient occlusion of the middle cerebral artery and 3 hours later randomly stereotaxically transplantated with 100K, 200K, or 400K human cerebral endothelial cell 6 viable cells or vehicle. Animals underwent neurological examination and motor test up to day 7 after transplantation then euthanized for immunostaining against neuronal, vascular, and specific human antigens. A parallel in vitro study cocultured rat primary neuronal cells with human cerebral endothelial cell 6 under

SUBMITTER: Ishikawa H 

PROVIDER: S-EPMC4083631 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature