Sequence-based appraisal of the genes encoding neck and carbohydrate recognition domain of conglutinin in blackbuck (Antilope cervicapra) and goat (Capra hircus).

ABSTRACT: Conglutinin, a collagenous C-type lectin, acts as soluble pattern recognition receptor (PRR) in recognition of pathogens. In the present study, genes encoding neck and carbohydrate recognition domain (NCRD) of conglutinin in goat and blackbuck were amplified, cloned, and sequenced. The obtained 488 bp ORFs encoding NCRD were submitted to NCBI with accession numbers KC505182 and KC505183. Both nucleotide and predicted amino acid sequences were analysed with sequences of other ruminants retrieved from NCBI GenBank using DNAstar and Megalign5.2 software. Sequence analysis revealed maximum similarity of blackbuck sequence with wild ruminants like nilgai and buffalo, whereas goat sequence displayed maximum similarity with sheep sequence at both nucleotide and amino acid level. Phylogenetic anal

SUBMITTER: Barik S 

PROVIDER: S-EPMC4083776 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature