Role of diacylglycerol activation of PKCθ in lipid-induced muscle insulin resistance in humans.

ABSTRACT: Muscle insulin resistance is a key feature of obesity and type 2 diabetes and is strongly associated with increased intramyocellular lipid content and inflammation. However, the cellular and molecular mechanisms responsible for causing muscle insulin resistance in humans are still unclear. To address this question, we performed serial muscle biopsies in healthy, lean subjects before and during a lipid infusion to induce acute muscle insulin resistance and assessed lipid and inflammatory parameters that have been previously implicated in causing muscle insulin resistance. We found that acute induction of muscle insulin resistance was associated with a transient increase in total and cytosolic diacylglycerol (DAG) content that was temporally associated with protein kinase (PKC)θ activation,

SUBMITTER: Szendroedi J 

PROVIDER: S-EPMC4084449 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature