Polymer-peptide delivery platforms: effect of oligopeptide orientation on polymer-based DNA delivery.

ABSTRACT: The success of nonviral transfection using polymers hinges on efficient nuclear uptake of nucleic acid cargo and overcoming intra- and extracellular barriers. By incorporating PKKKRKV heptapeptide pendent groups as nuclear localization signals (NLS) on a polymer backbone, we demonstrate protein expression levels higher than those obtained from JetPEI and Lipofectamine 2000, the latter being notorious for coupling high transfection efficiency with cytotoxicity. The orientation of the NLS peptide grafts markedly affected transfection performance. Polymers with the sequence attached to the backbone from the valine residue achieved a level of nuclear translocation higher than the levels of those having the NLS groups attached in the opposite orientation. The differences in nuclear localization

SUBMITTER: Parelkar SS 

PROVIDER: S-EPMC4084789 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature