Genome-wide analysis of HOXC9-induced neuronal differentiation of neuroblastoma cells.

ABSTRACT: Induction of differentiation is a therapeutic strategy in neuroblastoma, a common pediatric cancer of the sympathetic nervous system. The homeobox protein HOXC9 is a key regulator of neuroblastoma differentiation. To gain a molecular understanding of the function of HOXC9 in promoting differentiation of neuroblastoma cells, we conducted a genome-wide analysis of the HOXC9-induced differentiation program by microarray gene expression profiling and chromatin immunoprecipitation in combination with massively parallel sequencing (ChIP-seq). Here we describe in details the experimental system, methods, and quality control for the generation of the microarray and ChIP-seq data associated with our recent publication [1].

SUBMITTER: Wang X 

PROVIDER: S-EPMC4084856 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature