A kinetic study of the effects of light on circadian rhythmicity of the frq promoter of Neurospora crassa.

ABSTRACT: The role of the frq gene in the Neurospora crassa circadian rhythm has been widely studied, but technical limitations have hindered a thorough analysis of frq circadian expression waveform. Through our experiments, we have shown an improved precision in defining Neurospora's circadian rhythm kinetics using a codon optimized firefly luciferase gene reporter linked to a frq promoter. In vivo examination of this real-time reporter has allowed for a better understanding of the relationship of the light responsive elements of the frq promoter to its circadian feedback components. We provide a detailed phase response curve showing the phase shifts induced by a light pulse applied at different points of the circadian cycle. Using the frq-luc reporter, we have found that a 12-h light:12-h dark cyc

SUBMITTER: Gooch VD 

PROVIDER: S-EPMC4084941 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature