Regulation of serine protease inhibitor Kazal type-5 (SPINK5) gene expression in the keratinocytes.

ABSTRACT:

Objectives

Serine protease inhibitor Kazal type-5 (SPINK5) plays a crucial role in deciding the timing of desquamation of the skin. Its gene expression is limited at the very surface of the stratum granulosum (SG), whereas expression of kallikreins (KLKs) encoding proteases is usually found throughout the stratum spinosum and SG.

Methods

To explore the difference in expression regulation of these proteases/inhibitors, the function of SPINK5 promoter was examined using luciferase assay.

Results

Luciferase assay targeting the SPINK5 promoters (nucleotide -676/-532 and -318/-146 from the major transcription start site) showed high intensity in NHEK human keratinocyte. These two sites had neither common cis-elements nor GATA3 element but electrophoretic mobility shift ass

SUBMITTER: Le NA 

PROVIDER: S-EPMC4085257 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature