Targeting cells with single vectors using multiple-feature Boolean logic.

ABSTRACT: Precisely defining the roles of specific cell types is an intriguing frontier in the study of intact biological systems and has stimulated the rapid development of genetically encoded tools for observation and control. However, targeting these tools with adequate specificity remains challenging: most cell types are best defined by the intersection of two or more features such as active promoter elements, location and connectivity. Here we have combined engineered introns with specific recombinases to achieve expression of genetically encoded tools that is conditional upon multiple cell-type features, using Boolean logical operations all governed by a single versatile vector. We used this approach to target intersectionally specified populations of inhibitory interneurons in mammalian hippo

SUBMITTER: Fenno LE 

PROVIDER: S-EPMC4085277 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature