Expression profiles and initial confirmation of long noncoding RNAs in Chinese patients with pulmonary adenocarcinoma.

ABSTRACT:

Background

The purpose of this study was to investigate differentially expressed long noncoding RNAs (lncRNAs) in pulmonary adenocarcinoma tissue and adjacent noncancerous tissue from Chinese patients using lncRNA expression microarray and preliminary analysis.

Methods

RNA extracted from three paired pulmonary adenocarcinoma tissue and adjacent noncancerous tissue specimens was used to synthesize double-stranded complementary DNA after labeling and hybridization. The complementary DNA was labeled and hybridized to the lncRNA expression microarray, and array data were analyzed for hierarchical clustering. Gene coexpression networks were constructed to identify interactions among genes. To validate the microarray findings, we measured the relative expression levels of four ran

SUBMITTER: Zhao X 

PROVIDER: S-EPMC4085304 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature