Neuroendocrine-derived peptides promote prostate cancer cell survival through activation of IGF-1R signaling.

ABSTRACT:

Background

Neuroendocrine (NE) cells promote the progression of prostate cancer to a castration-resistant state through the production of paracrine growth factors. We have demonstrated this principle using in vitro and in vivo proliferative endpoints; however, the contributions of NE-derived pro-survival factors and anti-apoptosis to this phenomenon have not been thoroughly investigated.

Methods

Here, we utilized conditioned-medium (CM) from LNCaP cells, engineered to undergo NE differentiation, and examined its effects on PC3 and LNCaP cell survival.

Results

Statistically significant changes in clonogenic survival, Annexin V staining, PARP cleavage and trypan blue positivity of approximately twofold were observed in the presence of NE-derived CM relative to control-C

SUBMITTER: DaSilva JO 

PROVIDER: S-EPMC4085781 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature