RNAi screen identifies a synthetic lethal interaction between PIM1 overexpression and PLK1 inhibition.

ABSTRACT:

Purpose

To identify genes whose depletion is detrimental to Pim1-overexpressing prostate cancer cells and to validate this finding in vitro and in vivo.

Experimental design

RNAi screening was used to identify genes whose depletion is detrimental to Pim1-overexpressing cells. Our finding was validated using shRNA or PLK1-specific inhibitor BI 2536. Xenograft studies were performed using both PLK1-knockdown cells and BI 2536 to investigate the effects of PLK1 inhibition on tumorigenesis in Pim1-overexpressing cells. Finally, PLK1 and PIM1 expression patterns in human prostate tumors were examined by immunohistochemistry using tissue microarrays.

Results

We identified the mitotic regulator polo-like kinase (PLK1) as a gene whose depletion is particularly detrimental to t

SUBMITTER: van der Meer R 

PROVIDER: S-EPMC4086184 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature