Human immunodeficiency virus Tat associates with a specific set of cellular RNAs.

ABSTRACT:

Background

Human Immunodeficiency Virus 1 (HIV-1) exhibits a wide range of interactions with the host cell but whether viral proteins interact with cellular RNA is not clear. A candidate interacting factor is the trans-activator of transcription (Tat) protein. Tat is required for expression of virus genes but activates transcription through an unusual mechanism; binding to an RNA stem-loop, the transactivation response element (TAR), with the host elongation factor P-TEFb. HIV-1 Tat has also been shown to alter the expression of host genes during infection, contributing to viral pathogenesis but, whether Tat also interacts with cellular RNAs is unknown.

Results

Using RNA immunoprecipitation coupled with microarray analysis, we have discovered that HIV-1 Tat is associated wit

SUBMITTER: Bouwman RD 

PROVIDER: S-EPMC4086691 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature