Lactobacilli and bifidobacteria in human breast milk: influence of antibiotherapy and other host and clinical factors.

ABSTRACT:

Objective

The objective of this work was to study the lactobacilli and bifidobacteria population in human milk of healthy women, and to investigate the influence that several factors (including antibioteraphy during pregnancy and lactation, country and date of birth, delivery mode, or infant age) may exert on such population.

Methods

A total of 160 women living in Germany or Austria provided the breast milk samples. Initially, 66 samples were randomly selected and cultured on MRS-Cys agar plates. Then, the presence of DNA from the genera Lactobacillus and Bifidobacterium, and from most of the Lactobacillus and Bifidobacterium species that were isolated, was assessed by qualitative polymerase chain reaction (PCR) using genus- and species-specific primers.

Results

Lacto

SUBMITTER: Soto A 

PROVIDER: S-EPMC4086764 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature