Cellular localization, invasion, and turnover are differently influenced by healthy and tumor-derived extracellular matrix.

ABSTRACT: The interplay between tumor cells and the microenvironment has been recognized as one of the hallmarks of cancer biology. To assess the role of extracellular matrix (ECM) in the modulation of tissue homeostasis and tumorigenesis, we developed a protocol for the purification of tissue-derived ECM using mucosae from healthy human colon, perilesional area, and colorectal carcinoma (CRC). Matched specimens were collected from the left colon of patients undergoing CRC resection surgery. ECMs were obtained from tissues that were decellularized with hypotonic solutions containing ionic and nonionic detergents, hypertonic solution, and endonuclease in the absence of denaturing agents. Mucosae-derived ECMs maintained distribution and localization of proteins and glycoproteins typical of the origina

SUBMITTER: Genovese L 

PROVIDER: S-EPMC4086800 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature