Development of retroviral vectors for tissue-restricted expression in chicken embryonic gonads.

ABSTRACT: The chicken embryo has long been a useful model organism for studying development, including sex determination and gonadal differentiation. However, manipulating gene expression specifically in the embryonic avian gonad has been difficult. The viral vector RCASBP can be readily used for embryo-wide transgene expression; however global mis-expression using this method can cause deleterious off-target effects and embryo-lethality. In an attempt to develop vectors for the over-expression of sequences in chicken embryonic urogenital tissues, the viral vector RCANBP was engineered to contain predicted promoter sequences of gonadal-expressed genes. Several promoters were analysed and it was found that although the SF1 promoter produced a tissue-restricted expression pattern that was highest in t

SUBMITTER: Lambeth LS 

PROVIDER: S-EPMC4086957 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature