DNA barcodes for Nearctic Auchenorrhyncha (Insecta: Hemiptera).

ABSTRACT:

Background

Many studies have shown the suitability of sequence variation in the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene as a DNA barcode for the identification of species in a wide range of animal groups. We examined 471 species in 147 genera of Hemiptera: Auchenorrhyncha drawn from specimens in the Canadian National Collection of Insects to assess the effectiveness of DNA barcoding in this group.

Methodology/principal findings

Analysis of the COI gene revealed less than 2% intra-specific divergence in 93% of the taxa examined, while minimum interspecific distances exceeded 2% in 70% of congeneric species pairs. Although most species are characterized by a distinct sequence cluster, sequences for members of many groups of closely related species eith

SUBMITTER: Foottit RG 

PROVIDER: S-EPMC4087040 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature