Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs.

ABSTRACT:

Aim

To investigate the clinical significance and presence of mutations in the surface (S) and overlapping polymerase gene of hepatitis B patients with coexisting HBsAg and anti-HBs.

Methods

Twenty-three patients with chronic hepatitis B were studied. Of the 23 patients, 11 were both positive for hepatitis B virus (HBV) surface antigen (HBsAg) and antibody to HBV surface antigen (anti-HBs), 12 were negative for anti-HBs while positive for HBsAg. DNA was extracted from 200 muL serum of the patients. Nucleotide of the surface and overlapping polymerase gene from HBV-infected patients was amplified by PCR, and the PCR products were sequenced.

Results

Forty-one mutations were found within the surface gene protein of HBV in 15 patients (10 with coexisting HBsAg and anti-HBs

SUBMITTER: Lu HY 

PROVIDER: S-EPMC4087378 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature