Cloning and expression of SLC10A4, a putative organic anion transport protein.

ABSTRACT:

Aim

To determine if novel bile acid transporters may be expressed in human tissues.

Methods

SLC10A1 (NTCP) was used as a probe to search the NCBI database for homology to previously uncharacterized ESTs. The homology search identified an EST (termed SLC10A4) that shares sequence identity with SLC10A1 and SLC10A2 (ASBT). We performed Northern blot analysis and RT-PCR to determine the tissue distribution of SLC10A4. SLC10A4 was cloned in frame with an epitope tag and overexpressed in CHO cells to determine cellular localization and functional analysis of bile acid uptake.

Results

Northern analysis revealed that SLC10A4 mRNA is ubiquitously expressed in human tissues with the highest levels of mRNA expression in brain, placenta, and liver. In SLC10A4-transfected CHO cell

SUBMITTER: Splinter PL 

PROVIDER: S-EPMC4087434 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature