Differential effects on apoptosis induction in hepatocyte lines by stable expression of hepatitis B virus X protein.

ABSTRACT:

Aim

Hepatitis B virus protein X (HBx) has been shown to be weakly oncogenic in vitro. The transforming activities of HBx have been linked with the inhibition of several functions of the tumor suppressor p53. We have studied whether HBx may have different effects on p53 depending on the cell type.

Methods

We used the human hepatoma cell line HepG2 and the immortalized murine hepatocyte line AML12 and analyzed stably transfected clones which expressed physiological amounts of HBx. P53 was induced by UV irradiation.

Results

The p53 induction by UV irradiation was unaffected by stable expression of HBx. However, the expression of the cyclin kinase inhibitor p21(waf/cip/sdi) which gets activated by p53 was affected in the HBx transformed cell line AML12-HBx9, but not in He

SUBMITTER: Fiedler N 

PROVIDER: S-EPMC4087832 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature