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Reversing multidrug resistance by RNA interference through the suppression of MDR1 gene in human hepatoma cells.

ABSTRACT:

Aim

To reverse the multidrug resistance (MDR) by RNA interference (RNAi)-mediated MDR1 suppression in hepatoma cells.

Methods

For reversing MDR by RNAi technology, two different short hairpin RNAs (shRNAs) were designed and constructed into pGenSil-1 plasmid, respectively. They were then transfected into a highly adriamycin-resistant HepG2 hepatoma cell line (HepG2/ADM). The RNAi effect on MDR was evaluated by real-time PCR, cell cytotoxicity assay and rhodamine 123 (Rh123) efflux assy.

Results

The stably-transfected clones showed various degrees of reversal of MDR phenotype. Surprisingly, the MDR phenotype was completely reversed in two transfected clones.

Conclusion

MDR can be reversed by the shRNA-mediated MDRI suppression in HepG2/ADM cells, which provides a valuable clue to make multidrug-resistant hepatoma cells sensitive to anti-cancer drugs.

SUBMITTER: Chen XP

PROVIDER: S-EPMC4087887 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature

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Reversing multidrug resistance by RNA interference through the suppression of MDR1 gene in human hepatoma cells.

Chen Xiao-Ping XP Wang Qi Q Guan Jian J Huang Zhi-Yong ZY Zhang Wan-Guang WG Zhang Bi-Xiang BX

World journal of gastroenterology 20060601 21

<h4>Aim</h4>To reverse the multidrug resistance (MDR) by RNA interference (RNAi)-mediated MDR1 suppression in hepatoma cells.<h4>Methods</h4>For reversing MDR by RNAi technology, two different short hairpin RNAs (shRNAs) were designed and constructed into pGenSil-1 plasmid, respectively. They were then transfected into a highly adriamycin-resistant HepG2 hepatoma cell line (HepG2/ADM). The RNAi effect on MDR was evaluated by real-time PCR, cell cytotoxicity assay and rhodamine 123 (Rh123) efflux ...[more]

PMID: 16733848

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