Identification of differently expressed genes in human colorectal adenocarcinoma.

ABSTRACT:

Aim

To investigate the differently expressed genes in human colorectal adenocarcinoma.

Methods

The integrated approach for gene expression profiling that couples suppression subtractive hybridization, high-throughput cDNA array, sequencing, bioinformatics analysis, and reverse transcriptase real-time quantitative polymerase chain reaction (PCR) was carried out. A set of cDNA clones including 1260 SSH inserts amplified by PCR was arrayed using robotic printing. The cDNA arrays were hybridized with fluorescent-labeled probes prepared from RNA of human colorectal adenocarcinoma (HCRAC) and normal colorectal tissues.

Results

A total of 86 genes were identified, 16 unknown genes and 70 known genes. The transcription factor Somult9 influencing cell differentiation was downr

SUBMITTER: Chen Y 

PROVIDER: S-EPMC4087892 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature