Signaling pathways involved in the inhibition of epidermal growth factor receptor by erlotinib in hepatocellular cancer.

ABSTRACT:

Aim

To examine the underlying mechanisms of erlotinib-induced growth inhibition in hepatocellular carcinoma (HCC).

Methods

Erlotinib-induced alterations in gene expression were evaluated using cDNA array technology; changes in protein expression and/or protein activation due to erlotinib treatment as well as IGF-1-induced EGFR transactivation were investigated using Western blotting.

Results

Erlotinib treatment inhibited the mitogen activated protein (MAP)-kinase pathway and signal transducer of activation and transcription (STAT)-mediated signaling which led to an altered expression of apoptosis and cell cycle regulating genes as demonstrated by cDNA array technology. Overexpression of proapoptotic factors like caspases and gadds associated with a down-regulation of

SUBMITTER: Huether A 

PROVIDER: S-EPMC4088013 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature