Expression, purification and crystallization of the phosphate-binding PstS protein from Pseudomonas aeruginosa.

ABSTRACT: Pseudomonas aeruginosa (PA) infections pose a serious threat to human health. PA is a leading cause of fatal lung infections in cystic fibrosis and immune-suppressed patients, of sepsis in burn victims and of nosocomial infections. An important element in PA virulence is its ability to establish biofilms that evade suppression by the host's immune system and antibiotics. PstS, a periplasmic subunit of the Pst phosphate-transport system of PA, plays a critical role in the establishment of biofilms. In some drug-resistant PA strains, PstS is secreted in large quantities from the bacteria, where it participates in the assembly of adhesion fibres that enhance bacterial virulence. In order to understand the dual function of PstS in biofilm formation and phosphate transport, the crystal structur

SUBMITTER: Neznansky A 

PROVIDER: S-EPMC4089529 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature