Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.

ABSTRACT:

Background

Mass spectrometry-based proteomics enables the global identification and quantification of proteins and their posttranslational modifications in complex biological samples. However, proteomic analysis requires a complete and accurate reference set of proteins and is therefore largely restricted to model organisms with sequenced genomes.

Results

Here, we demonstrate the feasibility of deep genome-free proteomics by using a reference proteome derived from heterogeneous mRNA data. We identify more than 11,000 proteins with 99% confidence from the unfertilized Xenopus laevis egg and estimate protein abundance with approximately 2-fold precision. Our reference database outperforms the provisional gene models based on genomic DNA sequencing and references generated by o

SUBMITTER: Wuhr M 

PROVIDER: S-EPMC4090281 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature