TBLR1 is a novel prognostic marker and promotes epithelial-mesenchymal transition in cervical cancer.

ABSTRACT:

Background

Invasion and metastasis remain a critical issue in cervical cancer. However, the underlying mechanism of it in cervical cancer remains unclear. The newly discovered protein, TBLR1, plays a crucial role in regulating various key cellular functions.

Methods

In this study, western blot, real-time RT-PCR, immunohistochemical staining, 3D morphogenesis Matrigel culture, wound healing and Boyden chamber invasion assays, xenografted tumour model, luciferase assays, and chromatin immunoprecipitation assays were used.

Results

The expression of TBLR1 in cervical cancer cell lines and tissues was significantly upregulated at both the RNA and protein levels compared with that in normal cervical cells. Statistical analysis suggested that TBLR1 as an independent prognost

SUBMITTER: Wang J 

PROVIDER: S-EPMC4090741 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature