Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules.

ABSTRACT:

Background

DZIP1 (DAZ-interacting protein 1) has been described as a component of the Hh signaling pathway with a putative regulatory role in ciliogenesis. DZIP1 interacts with DAZ RNA binding proteins in embryonic stem cells and human germ cells suggesting a role in mRNA regulation.

Results

We investigated DZIP1 function in HeLa cells and its involvement in ribonucleoprotein complexes. DZIP1 was predominantly located in granules in the cytoplasm. Under oxidative stress conditions, DZIP1 re-localized to stress granules. DZIP appears to be important for the formation of stress granules during the stress response. We used immunoprecipitation assays with antibodies against DZIP1 and microarray hybridization to identify mRNAs associated with DZIP1. The genetic networks formed by

SUBMITTER: Shigunov P 

PROVIDER: S-EPMC4091656 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature