Cholesterol depletion induces transcriptional changes during skeletal muscle differentiation.

ABSTRACT:

Background

Myoblasts undergo major changes in their plasma membrane during the initial steps of skeletal muscle differentiation, including major alterations in the distribution of cholesterol. Cholesterol is involved in crucial membrane functions, such as fluidity, and permeability, and in the organization of specialized membrane microdomains (or lipid rafts). We have previously shown that alterations in cholesterol levels in myoblasts induce changes in proliferation and differentiation, which involves activation of Wnt/beta-catenin signaling pathway. In this study we used methyl-β-cyclodextrin (MbCD) to extract cholesterol from the membrane of chick skeletal muscle cells grown in culture. Using Ion Torrent-based sequencing, we compared the transcriptome of untreated and MbCD treat

SUBMITTER: Possidonio AC 

PROVIDER: S-EPMC4092213 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature