An integrated platform for bovine DNA methylome analysis suitable for small samples.

ABSTRACT:

Background

Oocytes and early embryos contain minute amounts of DNA, RNA and proteins, making the study of early mammalian development highly challenging. The study of the embryo epigenome, in particular the DNA methylome, has been made accessible thanks to the possibility of amplifying specific sequences according to their initial methylation status. This paper describes a novel platform dedicated to the genome-wide study of bovine DNA methylation, including a complete pipeline for data analysis and visualization. The platform allows processing and integrating of DNA methylome and transcriptome data from the same sample. Procedures were optimized for genome-wide analysis of 10 ng of DNA (10 bovine blastocysts). Bovine sperm and blastocysts were compared as a test of platform capabi

SUBMITTER: Shojaei Saadi HA 

PROVIDER: S-EPMC4092217 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature