Development of Genetic Markers for Triploid Verification of the Pacific Oyster, Crassostrea gigas.

ABSTRACT: The triploid Pacific oyster, which is produced by mating tetraploid and diploid oysters, is favored by the aquaculture industry because of its better flavor and firmer texture, particularly during the summer. However, tetraploid oyster production is not feasible in all oysters; the development of tetraploid oysters is ongoing in some oyster species. Thus, a method for ploidy verification is necessary for this endeavor, in addition to ploidy verification in aquaculture farms and in the natural environment. In this study, a method for ploidy verification of triploid and diploid oysters was developed using multiplex polymerase chain reaction (PCR) panels containing primers for molecular microsatellite markers. Two microsatellite multiplex PCR panels consisting of three markers each were devel

SUBMITTER: Kang JH 

PROVIDER: S-EPMC4093491 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature