Conserved features of the PB2 627 domain impact influenza virus polymerase function and replication.

ABSTRACT:

Unlabelled

Successful replication of influenza virus requires the coordinated expression of viral genes and replication of the genome by the viral polymerase, composed of the subunits PA, PB1, and PB2. Polymerase activity is regulated by both viral and host factors, yet the mechanisms of regulation and how they contribute to viral pathogenicity and tropism are poorly understood. To characterize these processes, we created a series of mutants in the 627 domain of the PB2 subunit. This domain contains a conserved "P[F/P]AAAPP" sequence motif and the well-described amino acid 627, whose identity regulates host range. A lysine present at position 627 in most mammalian viral isolates creates a basic face on the domain surface and confers high-level activity in humans compared to the glu

SUBMITTER: Kirui J 

PROVIDER: S-EPMC4093881 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature