The Dictyostelium discoideum RACK1 orthologue has roles in growth and development.

ABSTRACT:

Background

The receptor for activated C-kinase 1 (RACK1) is a conserved protein belonging to the WD40 repeat family of proteins. It folds into a beta propeller with seven blades which allow interactions with many proteins. Thus it can serve as a scaffolding protein and have roles in several cellular processes.

Results

We identified the product of the Dictyostelium discoideum gpbB gene as the Dictyostelium RACK1 homolog. The protein is mainly cytosolic but can also associate with cellular membranes. DdRACK1 binds to phosphoinositides (PIPs) in protein-lipid overlay and liposome-binding assays. The basis of this activity resides in a basic region located in the extended loop between blades 6 and 7 as revealed by mutational analysis. Similar to RACK1 proteins from other organis

SUBMITTER: Omosigho NN 

PROVIDER: S-EPMC4094278 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature